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Anti-Human BDNF Polyclonal Antibody
Anti-Human BDNF Polyclonal Antibody
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Обзор
Каталожный номерHB935014
Описание
Anti-BDNF Polyclonal Antibody (HB935014) is a rabbit polyclonal antibody detecting BDNF in ELISA, IF, IHC, WB. Suitable for Human, Mouse, Dog, Cat, and Ailurus fulgens.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IF, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human BDNF (Arg128-Arg247).
Цель ProBDNF, Abrineurin, Brain-derived neurotrophic factor, BDNF
Очистка Purified by antigen affinity column.
Номер доступа P23560
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Neurotrophic factor BDNF precursor form is a ~27 kDa protein. Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

1. Mowla, SJ. et al. (2001) The Journal of biological chemistry 276, 12660-6. PMID: 11152678
Примечание For research use only.
Изображения
  • Anti-Human BDNF Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-BDNF polyclonal antibody at 1µg/ml on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Mouse lung lysate
    Lane 3: Mouse cerebellum lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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