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Anti-BMP7 Polyclonal Antibody (HB664014)

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Vista general
Número de catálogoHB664014
Descripción
Anti-BMP7 Polyclonal Antibody (HB664014) is a rabbit polyclonal antibody detecting BMP7 in ELISA, IHC, WB. Suitable for Human, Mouse, and Dog.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human BMP7 (Asp30-His431).
Objetivo Bone morphogenetic protein 7, Eptotermin alfa, OP-1, OP1, BMP-7, Osteogenic protein 1, BMP7
Purificación Purified by antigen affinity column.
Número de acceso P18075
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Bone morphogenetic protein 7 (BMP7) is a ~49 kDa protein. Growth factor of the TGF-beta superfamily that plays important role in various biological processes, including embryogenesis, hematopoiesis, neurogenesis and skeletal morphogenesis. Initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2A. Once all three components are bound together in a complex at the cell surface, ACVR2A phosphorylates and activates ACVR1. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. For specific functions such as growth cone collapse in developing spinal neurons and chemotaxis of monocytes, also uses BMPR2 as type II receptor.

1. Perron, JC. et al. (2019) Biology open 8. PMID: 31208997
2. Greenwald, J. et al. (2003) Molecular cell 11, 605-17. PMID: 12667445
3. Macías-Silva, M. et al. (1998) The Journal of biological chemistry 273, 25628-36. PMID: 9748228
4. Groppe, J. et al. (2002) Nature 420, 636-42. PMID: 12478285
5. Brunmeir, R. et al. (2016) PLoS genetics 12, e1006474. PMID: 27923061
6. Arezes, J. et al. (2018) Blood 132, 1473-1477. PMID: 30097509
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-BMP7 Polyclonal Antibody

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