Anti-BRCC3 Polyclonal Antibody (HA695014)

Name: Anti-BRCC3 Polyclonal Antibody
Brand: abinScience
SKU: HA695014
Price: 168 USD
Availability: InStock

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Especificación:

50ug
100ug

Número:

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Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HA695014

Descripción

Anti-BRCC3 Polyclonal Antibody (HA695014) is a rabbit polyclonal antibody detecting Lys-63-specific deubiquitinase BRCC36; 3.4.19.-; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36; BRCC3; BRCC36; C6.1A; CXorf53 in ELISA, IHC, WB. Suitable for Human and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant human BRCC3 (Val12-Thr223).

Objetivo

Lys-63-specific deubiquitinase BRCC36; 3.4.19.-; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36; BRCC3; BRCC36; C6.1A; CXorf53

Nivel de endotoxinas

Please contact with the lab for this information.

Purificación

Purified by antigen affinity column.

Número de acceso

P46736, A5PJP6

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Lys-63-specific deubiquitinase BRCC36 is a ~36 kDa protein. Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates.

1. Cooper, EM. et al. (2009) The EMBO journal 28, 621-31. PMID: 19214193

2. Feng, L. et al. (2010) The Journal of biological chemistry 285, 30982-8. PMID: 20656690

3. Zheng, H. et al. (2013) Cell reports 5, 180-93. PMID: 24075985

4. Zeqiraj, E. et al. (2015) Molecular cell 59, 970-83. PMID: 26344097

5. Dong, Y. et al. (2003) Molecular cell 12, 1087-99. PMID: 14636569

6. Chen, X. et al. (2006) Cancer research 66, 5039-46. PMID: 16707425

7. Sobhian, B. et al. (2007) Science (New York, N.Y.) 316, 1198-202. PMID: 17525341

9. Shao, G. et al. (2009) Genes & development 23, 740-54. PMID: 19261746

10. Feng, L. et al. (2009) Genes & development 23, 719-28. PMID: 19261748

Nota

For research use only

Imágenes

Referencias

Datasheet

Anti-BRCC3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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