Anti-CACYBP Polyclonal Antibody (HV095014)

Name: Anti-CACYBP Polyclonal Antibody
Brand: abinScience
SKU: HV095014
Price: 96 USD
Availability: InStock

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Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HV095014

Descripción

Anti-CACYBP Polyclonal Antibody (HV095014) is a rabbit polyclonal antibody detecting CacyBP in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human CACYBP (Ala2-Phe228).

Objetivo

S100A6-binding protein, SIP, Siah-interacting protein, CacyBP, CACYBP, hCacyBP, S100A6BP, Calcyclin-binding protein

Purificación

Purified by antigen affinity column.

Número de acceso

Q9HB71

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Calcyclin-binding protein (CACYBP) is a ~26 kDa protein. May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).

Nota

For research use only.

Imágenes

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CACYBP (HV095014) at a concentration of 14.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CACYBP was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot

Western blot analysis was performed using anti-CACVBP polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: HCT116 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: 293T cell lysate
Lane 5: HepG2 cell lysate
Lane 6: HL60 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: Rat brain lysate
Lane 9: Rat liver lysate

Referencias

Datasheet

Anti-CACYBP Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo