Anti-CAPN1 Polyclonal Antibody (HC129014)

Name: Anti-CAPN1 Polyclonal Antibody
Brand: abinScience
SKU: HC129014
Price: 96 USD
Availability: InStock

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Especificación:

20ug
50ug
100ug

Número:

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Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HC129014

Descripción

Anti-CAPN1 Polyclonal Antibody (HC129014) is a rabbit polyclonal antibody detecting CAPN1 in ELISA, IHC, WB. Suitable for Human, Rat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant human CAPN1 (Gly27-Ser360).

Objetivo

Calpain 1, CANP 1, CANPL1, CAPN1, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 catalytic subunit, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, EC:3.4.22.52, Micromolar-calpain, muCANP

Nivel de endotoxinas

Please contact with the lab for this information.

Purificación

Purified by antigen affinity column.

Número de acceso

P07384, P97571, O35350

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Calpain-1 catalytic subunit (CAPN1) is a ~81 kDa protein. Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'. Cleaves and activates caspase-7 (CASP7).

1. Gafni, J. et al. (2009) The Journal of biological chemistry 284, 25441-9. PMID: 19617626

2. Hsu, CY. et al. (2011) The Journal of biological chemistry 286, 23222-33. PMID: 21531719

3. Sorimachi, H. et al. (1990) Biological chemistry Hoppe-Seyler 371 Suppl, 171-6. PMID: 2400579

4. Ono, Y. et al. (2013) Journal of molecular biology 425, 2955-72. PMID: 23707407

Nota

For research use only

Imágenes

Western blot

Western blot analysis was performed using anti-CAPN1 polyclonal antibody at 1ug/mL on recombinant CAPN1 (Catalog No. HC129012).

Referencias

Datasheet

Anti-CAPN1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo