Anti-CAPN1 Polyclonal Antibody (HC129014)

Name: Anti-CAPN1 Polyclonal Antibody
Brand: abinScience
SKU: HC129014
Price: 96 USD
Availability: InStock

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Aperçu
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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HC129014

Description

Anti-CAPN1 Polyclonal Antibody (HC129014) is a rabbit polyclonal antibody detecting CAPN1 in ELISA, IHC, WB. Suitable for Human, Rat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant human CAPN1 (Gly27-Ser360).

Cible

Calpain 1, CANP 1, CANPL1, CAPN1, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 catalytic subunit, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, EC:3.4.22.52, Micromolar-calpain, muCANP

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P07384, P97571, O35350

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Calpain-1 catalytic subunit (CAPN1) is a ~81 kDa protein. Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'. Cleaves and activates caspase-7 (CASP7).

1. Gafni, J. et al. (2009) The Journal of biological chemistry 284, 25441-9. PMID: 19617626

2. Hsu, CY. et al. (2011) The Journal of biological chemistry 286, 23222-33. PMID: 21531719

3. Sorimachi, H. et al. (1990) Biological chemistry Hoppe-Seyler 371 Suppl, 171-6. PMID: 2400579

4. Ono, Y. et al. (2013) Journal of molecular biology 425, 2955-72. PMID: 23707407

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-CAPN1 polyclonal antibody at 1ug/mL on recombinant CAPN1 (Catalog No. HC129012).

Reférences

Fiche technique

Anti-CAPN1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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