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Anti-CAPN1 Polyclonal Antibody
Anti-CAPN1 Polyclonal Antibody
Цена(USD):
Спецификация:
  • 20ug
  • 50ug
  • 100ug
Количество:
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Обзор
Каталожный номерHC129014
Описание
Anti-CAPN1 Polyclonal Antibody (HC129014) is a rabbit polyclonal antibody detecting CAPN1 in ELISA, IHC, WB. Suitable for Human, Rat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant human CAPN1 (Gly27-Ser360).
Цель Calpain 1, CANP 1, CANPL1, CAPN1, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 catalytic subunit, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, EC:3.4.22.52, Micromolar-calpain, muCANP
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа P07384, P97571, O35350
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Calpain-1 catalytic subunit (CAPN1) is a ~81 kDa protein. Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'. Cleaves and activates caspase-7 (CASP7).

1. Gafni, J. et al. (2009) The Journal of biological chemistry 284, 25441-9. PMID: 19617626
2. Hsu, CY. et al. (2011) The Journal of biological chemistry 286, 23222-33. PMID: 21531719
3. Sorimachi, H. et al. (1990) Biological chemistry Hoppe-Seyler 371 Suppl, 171-6. PMID: 2400579
4. Ono, Y. et al. (2013) Journal of molecular biology 425, 2955-72. PMID: 23707407
Примечание For research use only
Изображения
  • Anti-CAPN1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CAPN1 polyclonal antibody at 1ug/mL on recombinant CAPN1 (Catalog No. HC129012).

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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