

| カタログ番号 | HY178014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-CAPNS1 Polyclonal Antibody (HY178014) is a rabbit polyclonal antibody detecting CAPNS1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pig.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human CAPNS1 (Met1-Ser268). | ||||||||
| ターゲット | CAPN4, CAPNS1, Calpain regulatory subunit, Calcium-dependent protease small subunit 1, Calcium-dependent protease small subunit, Calcium-activated neutral proteinase small subunit, CDPS, CSS1, Calpain small subunit 1, CANP small subunit, CAPNS | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P04632 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Calpain small subunit 1 (CAPNS1) is a ~28 kDa protein. Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Essential for embryonic development. | ||||||||
| 注意事項 | For research use only. |

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CAPNS1 (HY178014) at a concentration of 16 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CAPNS1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).


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