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Anti-Capsid Polyclonal Antibody (VK719014)

Anti-Capsid Polyclonal Antibody
Anti-Capsid Polyclonal Antibody
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  • 100ug
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概要
カタログ番号VK719014
説明
Anti-Capsid Polyclonal Antibody (VK719014) is a rabbit polyclonal antibody detecting Capsid protein C in ELISA, IHC, WB. Suitable for Dengue virus type 1 and Dengue virus type 3.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
種反応性Dengue virus 1 (DENV-1)
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Capsid (Asn21-Arg100).
ターゲット Genome polyprotein, Capsid protein C, Capsid protein, Core protein, Protein prM, Precursor membrane protein, Peptide pr, Peptide precursor, Small envelope protein M, Matrix protein, Envelope protein E, Non-structural protein 1, NS1, Non-structural protein 2A, NS2A, Serine protease subunit NS2B, Flavivirin protease NS2B regulatory subunit, Non-structural protein 2B, Serine protease NS3, EC:3.4.21.91, EC:3.6.1.15, EC:3.6.4.13, Flavivirin protease NS3 catalytic subunit, Non-structural protein 3, Non-structural protein 4A, NS4A, Peptide 2k, Non-structural protein 4B, NS4B, RNA-directed RNA polymerase NS5, EC:2.1.1.56, EC:2.1.1.57, EC:2.7.7.48, Non-structural protein 5
エンドトキシンレベル Please contact with the lab for this information.
精製 Purified by antigen affinity column.
アクセッション番号 P17763, P33478, P27913, P27909, P27912, Q6YMS3, Q6YMS4, Q99D35, P27915, Q5UB51
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Genome polyprotein (Capsid) is a ~378 kDa protein. Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA (Probable). Plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions.

1. Kuhn, RJ. et al. (2002) Cell 108, 717-25. PMID: 11893341
2. Sangiambut, S. et al. (2008) The Journal of general virology 89, 1254-1264. PMID: 18420804
3. Colpitts, TM. et al. (2011) PloS one 6, e24365. PMID: 21909430
4. Bhuvanakantham, R. et al. (2013) Cellular microbiology 15, 1688-706. PMID: 23522008
注意事項 For research use only
イメージ
  • Anti-Capsid Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NENV-1 Capsid polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant DENV-1 Capsid protein C/Core protein (Catalog No: VK719012)

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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