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Anti-CASC3 Polyclonal Antibody (HA754014)

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Aperçu
Numéro de catalogueHA754014
Description
Anti-CASC3 Polyclonal Antibody (HA754014) is a rabbit polyclonal antibody detecting Protein CASC3; Cancer susceptibility candidate gene 3 protein; Metastatic lymph node gene 51 protein; MLN 51; Protein barentsz; Btz; CASC3; MLN51 in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant human CASC3 (Ala69-Lys260).
Cible Protein CASC3; Cancer susceptibility candidate gene 3 protein; Metastatic lymph node gene 51 protein; MLN 51; Protein barentsz; Btz; CASC3; MLN51
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès O15234, A5D7H5, Q8K3W3, Q8K3X0
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Protein CASC3 is a ~76 kDa protein. Required for pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Stimulates the ATPase and RNA-helicase activities of EIF4A3.

1. Zhang, X. et al. (2017) Cell 169, 918-929.e14. PMID: 28502770
2. Zhan, X. et al. (2018) Science (New York, N.Y.) 359, 537-545. PMID: 29301961
Note For research use only
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Reférences
Fiche technique
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-CASC3 Polyclonal Antibody

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