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| カタログ番号 | HB991014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-CASP1/Caspase-1 Polyclonal Antibody (HB991014) is a rabbit polyclonal antibody detecting Caspase-1 in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human CASP1/Caspase-1 (Asn120-Asp297). | ||||||||
| ターゲット | IL1BC, Interleukin-1 beta-converting enzyme, p45, Caspase-1, IL-1BC, IL-1 beta-converting enzyme, CASP1, IL1BCE, ICE, CASP-1, Interleukin-1 beta convertase | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P29466 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Caspase-1 (CASP1) is a ~45 kDa protein. Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukin IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. 1. Gaggero, A. et al. (2004) Oncogene 23, 7552-60. PMID: 15326478 2. Feng, Q. et al. (2004) Genomics 84, 587-91. PMID: 15498465 3. Thornberry, NA. et al. (1992) Nature 356, 768-74. PMID: 1574116 4. Shi, J. et al. (2015) Nature 526, 660-5. PMID: 26375003 5. Ball, DP. et al. (2020) Life science alliance 3. PMID: 32051255 6. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714 7. Alnemri, ES. et al. (1995) The Journal of biological chemistry 270, 4312-7. PMID: 7876192 8. Akita, K. et al. (1997) The Journal of biological chemistry 272, 26595-603. PMID: 9334240 9. Wang, K. et al. (2020) Cell 180, 941-955.e20. PMID: 32109412 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-CASP8 polyclonal antibody at 1ug/mL on various samples.
Lane 1: THP-1 cell lysate
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