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Anti-CASP14 Polyclonal Antibody (HB268014)

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Aperçu
Numéro de catalogueHB268014
Description
Anti-CASP14 Polyclonal Antibody (HB268014) is a rabbit polyclonal antibody detecting CASP14 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human CASP14 (Met1-Gln242).
Cible CASP14, CASP-14, Caspase-14
Purification Purified by antigen affinity column.
Numéro d'accès P31944
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Caspase-14 (CASP14) is a ~27 kDa protein. Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum. Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin. In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF.

1. Fischer, H. et al. (2004) FEBS letters 577, 446-50. PMID: 15556625
2. Park, K. et al. (2006) Biochemical and biophysical research communications 347, 941-8. PMID: 16854378
3. Hibino, T. et al. (2010) Journal of cellular biochemistry 109, 487-97. PMID: 19960512
4. Beasley, S. et al. (2014) BioMed research international 2014, 417986. PMID: 25121097
5. Yamamoto-Tanaka, M. et al. (2014) Cell death & disease 5, e1181. PMID: 24743736
Note For research use only.
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Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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