Anti-CASP4 Polyclonal Antibody (HW474014)

Name: Anti-CASP4 Polyclonal Antibody
Brand: abinScience
SKU: HW474014
Price: 168 USD
Availability: InStock

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概要
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参考文献
データシート
SDS

概要

カタログ番号

HW474014

説明

Anti-CASP4 Polyclonal Antibody (HW474014) is a rabbit polyclonal antibody detecting CASP4 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

種反応性

Human

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human CASP4 (Asn78-Glu265).

ターゲット

Mih1, ICH2, CASP-4, ICE and Ced-3 homolog 2, CASP4, Protease TX, Caspase-4, ICE(rel)-II, ICH-2

精製

Purified by antigen affinity column.

アクセッション番号

P49662

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Caspase-4 (CASP4) is a ~43 kDa protein. Inflammatory caspase that acts as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (LPS)-induced pyroptosis. Also indirectly activates the NLRP3 and NLRP6 inflammasomes. Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS, GSDMD and IL18. Effector of the non-canonical inflammasome independently of NLRP3 inflammasome and CASP1: the non-canonical inflammasome promotes pyroptosis through GSDMD cleavage without involving secretion of cytokine IL1B. In the non-canonical inflammasome, CASP4 is activated by direct binding to the lipid A moiety of LPS without the need of an upstream sensor.

1. Shi, J. et al. (2014) Nature 514, 187-92. PMID: 25119034

2. Shi, J. et al. (2015) Nature 526, 660-5. PMID: 26375003

3. Wang, K. et al. (2020) Cell 180, 941-955.e20. PMID: 32109412

4. Li, Z. et al. (2021) Nature 599, 290-295. PMID: 34671164

5. Zhu, F. et al. (2023) Immunity 56, 753-767.e8. PMID: 37001519

6. Devant, P. et al. (2023) Nature 624, 451-459. PMID: 37993712

7. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714

8. Blasche, S. et al. (2013) PloS one 8, e58937. PMID: 23516580

10. Gaggero, A. et al. (2004) Oncogene 23, 7552-60. PMID: 15326478

注意事項

For research use only.

イメージ

参考文献

データシート

Anti-CASP4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-CASP4 Polyclonal Antibody (HW474014)

概要

イメージ

参考文献

データシート

SDS