Anti-CASP8 Polyclonal Antibody (HC266014)

Name: Anti-CASP8 Polyclonal Antibody
Brand: abinScience
SKU: HC266014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Número de catálogo

HC266014

Descripción

Anti-CASP8 Polyclonal Antibody (HC266014) is a rabbit polyclonal antibody detecting CASP8 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human CASP8 (Ser217-Asp479).

Objetivo

CAP4, MCH5, FADD-homologous ICE/ced-3-like protease, FLICE, Apoptotic cysteine protease, MORT1-associated ced-3 homolog, MACH, FADD-like ICE, ICE-like apoptotic protease 5, CASP8, CASP-8, Caspase-8, Apoptotic protease Mch-5

Purificación

Purified by antigen affinity column.

Número de acceso

Q14790

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Caspase-8 (CASP8) is a ~55 kDa protein. Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. Binding to the adapter molecule FADD recruits it to either receptor FAS/TNFRSF6 or TNFRSF1A. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation.

1. Blasche, S. et al. (2013) PloS one 8, e58937. PMID: 23516580

2. Peng, T. et al. (2022) Molecular cell 82, 1806-1820.e8. PMID: 35338844

3. Liu, Y. et al. (2022) mBio 13, e0069022. PMID: 35446120

4. Boldin, MP. et al. (1996) Cell 85, 803-15. PMID: 8681376

5. Muzio, M. et al. (1996) Cell 85, 817-27. PMID: 8681377

6. Srinivasula, SM. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 14486-91. PMID: 8962078

7. Muzio, M. et al. (1997) The Journal of biological chemistry 272, 2952-6. PMID: 9006941

8. Medema, JP. et al. (1997) The EMBO journal 16, 2794-804. PMID: 9184224

9. Denault, JB. et al. (2006) Molecular cell 23, 523-33. PMID: 16916640

10. Tao, P. et al. (2020) Nature 577, 109-114. PMID: 31827280

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-CASP8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-CASP8 Polyclonal Antibody (HC266014)

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Imágenes

Referencias

Datasheet

SDS