Anti-CASP8 Polyclonal Antibody (HC266014)

Name: Anti-CASP8 Polyclonal Antibody
Brand: abinScience
SKU: HC266014
Price: 168 USD
Availability: InStock

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50ug
100ug

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SDS

Aperçu

Numéro de catalogue

HC266014

Description

Anti-CASP8 Polyclonal Antibody (HC266014) is a rabbit polyclonal antibody detecting CASP8 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human CASP8 (Ser217-Asp479).

Cible

CAP4, MCH5, FADD-homologous ICE/ced-3-like protease, FLICE, Apoptotic cysteine protease, MORT1-associated ced-3 homolog, MACH, FADD-like ICE, ICE-like apoptotic protease 5, CASP8, CASP-8, Caspase-8, Apoptotic protease Mch-5

Purification

Purified by antigen affinity column.

Numéro d'accès

Q14790

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Caspase-8 (CASP8) is a ~55 kDa protein. Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. Binding to the adapter molecule FADD recruits it to either receptor FAS/TNFRSF6 or TNFRSF1A. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation.

1. Blasche, S. et al. (2013) PloS one 8, e58937. PMID: 23516580

2. Peng, T. et al. (2022) Molecular cell 82, 1806-1820.e8. PMID: 35338844

3. Liu, Y. et al. (2022) mBio 13, e0069022. PMID: 35446120

4. Boldin, MP. et al. (1996) Cell 85, 803-15. PMID: 8681376

5. Muzio, M. et al. (1996) Cell 85, 817-27. PMID: 8681377

6. Srinivasula, SM. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 14486-91. PMID: 8962078

7. Muzio, M. et al. (1997) The Journal of biological chemistry 272, 2952-6. PMID: 9006941

8. Medema, JP. et al. (1997) The EMBO journal 16, 2794-804. PMID: 9184224

9. Denault, JB. et al. (2006) Molecular cell 23, 523-33. PMID: 16916640

10. Tao, P. et al. (2020) Nature 577, 109-114. PMID: 31827280

Note

For research use only.

Images

Reférences

Fiche technique

Anti-CASP8 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-CASP8 Polyclonal Antibody (HC266014)

Aperçu

Images

Reférences

Fiche technique

SDS