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Anti-CBR3 Polyclonal Antibody (HF500014)

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Anti-CBR3 Polyclonal Antibody
Anti-CBR3 Polyclonal Antibody
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Aperçu
Numéro de catalogueHF500014
Description
Anti-CBR3 Polyclonal Antibody (HF500014) is a rabbit polyclonal antibody detecting CBR3 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human CBR3 (Met1-Trp277).
Cible Carbonyl reductase [NADPH] 3, Short chain dehydrogenase/reductase family 21C member 2, NADPH-dependent carbonyl reductase 3, CBR3
Purification Purified by antigen affinity column.
Numéro d'accès O75828
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Carbonyl reductase [NADPH] 3 (CBR3) is a ~30 kDa protein. Catalyzes the NADPH-dependent reduction of carbonyl compounds to their corresponding alcohols. Has low NADPH-dependent oxidoreductase activity. Acts on several orthoquinones, acts as well on non-quinone compounds, such as isatin or on the anticancer drug oracin. Best substrates for CBR3 is 1,2- naphthoquinone, hence could play a role in protection against cytotoxicity of exogenous quinones. Exerts activity toward ortho-quinones but not paraquinones.

1. Miura, T. et al. (2008) Molecular and cellular biochemistry 315, 113-21. PMID: 18493841
2. Lakhman, SS. et al. (2005) Drug metabolism and disposition: the biological fate of chemicals 33, 254-7. PMID: 15537833
3. Pilka, ES. et al. (2009) PloS one 4, e7113. PMID: 19841672
Note For research use only.
Images
  • Anti-CBR3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CBR3 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: MCF7 cell lysate
    Lane 2: Mouse ovary lysate
    Lane 3: Mouse uterus lysate

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-CBR3 Polyclonal Antibody

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