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Anti-Human CD106/VCAM1 Polyclonal Antibody (HB032014)

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Vista general
Número de catálogoHB032014
Descripción
Anti-CD106/VCAM1 Polyclonal Antibody (HB032014) is a rabbit polyclonal antibody detecting VCAM1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human CD106/VCAM1 (Phe25-Glu698).
Objetivo Vascular cell adhesion protein 1, VCAM1, CD106, INCAM-100, V-CAM 1, VCAM-1
Purificación Purified by antigen affinity column.
Número de acceso P19320
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Vascular cell adhesion protein 1 (CD106/VCAM1) is a ~81 kDa protein. Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation.

1. Li, Y. et al. (2020) Journal of molecular cell biology 12, 190-201. PMID: 31310649
2. Taooka, Y. et al. (1999) The Journal of cell biology 145, 413-20. PMID: 10209034
3. Marchese, ME. et al. (2012) Biochemistry 51, 8235-46. PMID: 22970700
4. Pinho, S. et al. (2022) Nature cell biology 24, 290-298. PMID: 35210567
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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