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Anti-Human Band 3 Polyclonal Antibody (HF626024)

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Vista general
Número de catálogoHF626024
Descripción
Anti-CD233/SLC4A1 Polyclonal Antibody (HF626024) is a rabbit polyclonal antibody detecting SLC4A1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant CD233/SLC4A1 (3x(Lys542-Thr570&Ile624-Met663)).
Objetivo AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, Band 3 anion transport protein, CD233, DI, EPB3, SLC4A1, Solute carrier family 4 member 1
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Purified by antigen affinity column.
Número de acceso P02730
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Band 3 anion transport protein (CD233/SLC4A1) is a ~101 kDa protein. Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Component of the ankyrin-1 complex of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.

1. Bruce, LJ. et al. (2000) The Biochemical journal 350 Pt 1, 41-51. PMID: 10926824
2. Rungroj, N. et al. (2004) The Journal of biological chemistry 279, 13833-8. PMID: 14734552
3. Schofield, AE. et al. (1992) Journal of molecular biology 223, 949-58. PMID: 1538405
4. Bruce, LJ. et al. (2005) Nature genetics 37, 1258-63. PMID: 16227998
5. Ungsupravate, D. et al. (2010) Molecular membrane biology 27, 92-103. PMID: 20151848
6. Shnitsar, V. et al. (2013) The Journal of biological chemistry 288, 33848-33860. PMID: 24121512
7. Genetet, S. et al. (2017) Scientific reports 7, 46170. PMID: 28387307
8. Vallese, F. et al. (2022) Nature structural & molecular biology 29, 706-718. PMID: 35835865
Nota For research use only.
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human Band 3 Polyclonal Antibody

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