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Anti-Human Neuropilin-1/NRP1 Polyclonal Antibody (HT070014)

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Aperçu
Numéro de catalogueHT070014
Description
Anti-CD304/NRP1 Polyclonal Antibody (HT070014) is a rabbit polyclonal antibody detecting CD304 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human CD304/NRP1 (Lys274-Glu584).
Cible NRP1, Neuropilin-1, VEGF165R, NRP, CD304, Vascular endothelial cell growth factor 165 receptor
Purification Purified by antigen affinity column.
Numéro d'accès O14786
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Neuropilin-1 (CD304/NRP1) is a ~103 kDa protein. Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins. Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis.

1. Gagnon, ML. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 2573-8. PMID: 10688880
2. He, Z. et al. (1997) Cell 90, 739-51. PMID: 9288753
3. Soker, S. et al. (1998) Cell 92, 735-45. PMID: 9529250
4. Teesalu, T. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 16157-62. PMID: 19805273
5. Issitt, T. et al. (2019) iScience 11, 205-223. PMID: 30623799
Note For research use only.
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Formula
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Working Solution
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Anti-Human Neuropilin-1/NRP1 Polyclonal Antibody

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