Anti-Human EpCAM/TROP1 Polyclonal Antibody (HB104014)

Name: Anti-Human EpCAM/TROP1 Polyclonal Antibody
Brand: abinScience
SKU: HB104014
Price: 96 USD
Availability: InStock

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Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HB104014

Descripción

Anti-CD326/EPCAM Polyclonal Antibody (HB104014) is a rabbit polyclonal antibody detecting EPCAM in ELISA, IHC, WB, IF. Suitable for Human and Macaca mulatta.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB, IF

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human CD326/EPCAM (Gln24-Lys265).

Aplicaciones probadas

Objetivo

Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1, EGP, EGP314, EPCAM, Ep-CAM, Epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TACSTD1, TROP1, Tumor-associated calcium signal transducer 1, hEGP314

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>90% as determined by SDS-PAGE.

Purificación

Purified by antigen affinity column.

Número de acceso

P16422

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Epithelial cell adhesion molecule (CD326/EPCAM) is a ~34 kDa protein. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

Nota

For research use only.

Imágenes

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting EpCAM (HB104014) at a concentration of 20.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of EpCAM was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot

Western blot analysis was performed using anti-EPCAM polyclonal antibody at 1μg/ml on various samples.
Lane 1: HCT116 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HT-29 cell lysate
Lane 4: Mouse colon lysate