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| カタログ番号 | HB104014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-CD326/EPCAM Polyclonal Antibody (HB104014) is a rabbit polyclonal antibody detecting EPCAM in ELISA, IHC, WB, IF. Suitable for Human and Macaca mulatta.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB, IF | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human CD326/EPCAM (Gln24-Lys265). | ||||||||
| テスト済みアプリケーション | WB | ||||||||
| ターゲット | Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1, EGP, EGP314, EPCAM, Ep-CAM, Epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TACSTD1, TROP1, Tumor-associated calcium signal transducer 1, hEGP314 | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 純度 | >90% as determined by SDS-PAGE. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P16422 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Epithelial cell adhesion molecule (CD326/EPCAM) is a ~34 kDa protein. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. | ||||||||
| 注意事項 | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting EpCAM (HB104014) at a concentration of 20.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of EpCAM was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot analysis was performed using anti-EPCAM polyclonal antibody at 1μg/ml on various samples.
Lane 1: HCT116 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HT-29 cell lysate
Lane 4: Mouse colon lysate
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