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Anti-CDC20 Polyclonal Antibody (HC333014)

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Aperçu
Numéro de catalogueHC333014
Description
Anti-CDC20 Polyclonal Antibody (HC333014) is a rabbit polyclonal antibody detecting CDC20 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human CDC20 (Gln218-Arg471).
Cible Cell division cycle protein 20 homolog, p55CDC, CDC20
Purification Purified by antigen affinity column.
Numéro d'accès Q12834
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Cell division cycle protein 20 homolog (CDC20) is a ~54 kDa protein. Substrate-specific adapter of the anaphase promoting complex/cyclosome (APC/C) complex that confers substrate specificity by binding to substrates and targeting them to the APC/C complex for ubiquitination and degradation. Recognizes and binds the destruction box (D box) on protein substrates. Involved in the metaphase/anaphase transition of cell cycle. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons.

1. Fang, G. et al. (1998) Molecular cell 2, 163-71. PMID: 9734353
2. Lin, Z. et al. (2015) Cell discovery 1, 15028-. PMID: 27030811
3. Choppara, S. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 998-1003. PMID: 29343641
4. Zhao, L. et al. (2020) Protein & cell 11, 921-927. PMID: 32666501
5. Kramer, ER. et al. (1998) Current biology : CB 8, 1207-10. PMID: 9811605
6. Fang, G. et al. (1998) Genes & development 12, 1871-83. PMID: 9637688
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-CDC20 Polyclonal Antibody

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