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Anti-CDC42 Polyclonal Antibody (HX069014)

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Aperçu
Numéro de catalogueHX069014
Description
Anti-CDC42 Polyclonal Antibody (HX069014) is a rabbit polyclonal antibody detecting CDC42 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Aedes aegypti.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human CDC42 (Met1-Cys188).
Cible CDC42, G25K GTP-binding protein, Cell division control protein 42 homolog
Purification Purified by antigen affinity column.
Numéro d'accès P60953
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Cell division control protein 42 homolog (CDC42) is a ~21 kDa protein. Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Regulates cell migration.

1. Oceguera-Yanez, F. et al. (2005) The Journal of cell biology 168, 221-32. PMID: 15642749
2. Modzelewska, K. et al. (2006) The Journal of biological chemistry 281, 37527-35. PMID: 17038317
3. Hong, IK. et al. (2012) The Journal of biological chemistry 287, 32027-39. PMID: 22843693
4. Gauthier-Campbell, C. et al. (2004) Molecular biology of the cell 15, 2205-17. PMID: 14978216
5. Majmundar, AJ. et al. (2021) Science advances 7. PMID: 33523862
6. Schlam, D. et al. (2015) Nature communications 6, 8623. PMID: 26465210
7. Ninomiya, K. et al. (2021) Journal of cell science 134. PMID: 33310911
Note For research use only.
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Reférences
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-CDC42 Polyclonal Antibody

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