

| Каталожный номер | JN085033 | ||||
|---|---|---|---|---|---|
| Реактивность видов | Clostridioides difficile | ||||
| Применения | ELISA, Neutralization | ||||
| Хозяинский вид | Human | ||||
| Клоональность | Monoclonal | ||||
| Изотип | IgG1, kappa | ||||
| ID клона | SAA1378 | ||||
| Цель | Toxin A, 3.4.22.-, Glucosyltransferase TcdA, 2.4.1.-, tcdA, toxA | ||||
| Уровень эндотоксинов | Please contact with the lab for this information. | ||||
| Чистота | >95% as determined by SDS-PAGE. | ||||
| Очистка | Protein A/G purified from cell culture supernatant. | ||||
| Номер доступа | P16154 | ||||
| RRID | Anti-C.difficile Toxin A/tcdA Antibody (SAA1378) (abinScience Cat# JN085033, RRID:AB_3726896) |
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| Форма | Liquid | ||||
| Буфер хранения | 0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
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| Информация об использовании продукта |
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| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| Фон | Toxin A (tcdA) is a ~308 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdA and TcdB constitute the main toxins that mediate the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdB, TcdA is less virulent and less important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdA) into the host cytosol. Targets colonic epithelia by binding to some receptor, and enters host cells via clathrin-mediated endocytosis. _x005F_x005F_x000D_ _x005F_x005F_x000D_1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489 _x005F_x005F_x000D_
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2. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482 _x005F_x005F_x000D_
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3. Greco, A. et al. (2006) Nature structural & molecular biology 13, 460-1. PMID: 16622409 _x005F_x005F_x000D_
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4. Tucker, KD. et al. (1991) Infection and immunity 59, 73-8. PMID: 1670930 _x005F_x005F_x000D_
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5. Tao, L. et al. (2019) Nature microbiology 4, 1760-1769. PMID: 31160825 _x005F_x005F_x000D_
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6. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706 _x005F_x005F_x000D_
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7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356 _x005F_x005F_x000D_
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8. Pruitt, RN. et al. (2009) The Journal of biological chemistry 284, 21934-21940. PMID: 19553670 | ||||
| Примечание | For research use only. |

SDS-PAGE for Anti-C.difficile Toxin A/tcdA Antibody (SAA1378).




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