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Anti-C.difficile Toxin A/tcdA Recombinant Antibody (SAA1378) (JN085033)

Anti-C.difficile Toxin A/tcdA Recombinant Antibody (SAA1378)
Anti-C.difficile Toxin A/tcdA Recombinant Antibody (SAA1378)
Цена(USD):
Спецификация:
  • 20ug
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерJN085033
Реактивность видовClostridioides difficile
ПримененияELISA, Neutralization
Хозяинский видHuman
КлоональностьMonoclonal
ИзотипIgG1, kappa
ID клонаSAA1378
Цель Toxin A, 3.4.22.-, Glucosyltransferase TcdA, 2.4.1.-, tcdA, toxA
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >95% as determined by SDS-PAGE.
Очистка Protein A/G purified from cell culture supernatant.
Номер доступа P16154
RRID

Anti-C.difficile Toxin A/tcdA Antibody (SAA1378) (abinScience Cat# JN085033, RRID:AB_3726896)
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Форма Liquid
Буфер хранения 0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:2000-1:20000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Фон

Toxin A (tcdA) is a ~308 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdA and TcdB constitute the main toxins that mediate the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdB, TcdA is less virulent and less important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdA) into the host cytosol. Targets colonic epithelia by binding to some receptor, and enters host cells via clathrin-mediated endocytosis.

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1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489
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2. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482
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3. Greco, A. et al. (2006) Nature structural & molecular biology 13, 460-1. PMID: 16622409
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4. Tucker, KD. et al. (1991) Infection and immunity 59, 73-8. PMID: 1670930
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5. Tao, L. et al. (2019) Nature microbiology 4, 1760-1769. PMID: 31160825
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6. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706
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7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356
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8. Pruitt, RN. et al. (2009) The Journal of biological chemistry 284, 21934-21940. PMID: 19553670
Примечание For research use only.
Изображения
  • Anti-C.difficile Toxin A/tcdA Recombinant Antibody (SAA1378)

    SDS-PAGE

    SDS-PAGE for Anti-C.difficile Toxin A/tcdA Antibody (SAA1378).

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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