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Обзор
Каталожный номерHB532014
Описание
Anti-CDK10 Polyclonal Antibody (HB532014) is a rabbit polyclonal antibody detecting Cyclin-dependent kinase 10; 2.7.11.22; Cell division protein kinase 10; Serine/threonine-protein kinase PISSLRE; CDK10 in ELISA, IHC, WB. Suitable for Human, Rat, Mouse, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant human CDK10 (Met1-Pro360).
Цель Cyclin-dependent kinase 10; 2.7.11.22; Cell division protein kinase 10; Serine/threonine-protein kinase PISSLRE; CDK10
Уровень эндотоксинов Please contact with the lab for this information.
Очистка Purified by antigen affinity column.
Номер доступа Q15131, Q4KM47, Q3UMM4, Q2TBL8
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Cyclin-dependent kinase 10 (CDK10) is a ~41 kDa protein. Cyclin-dependent kinase that phosphorylates the transcription factor ETS2 (in vitro) and positively controls its proteasomal degradation (in cells). Involved in the regulation of actin cytoskeleton organization through the phosphorylation of actin dynamics regulators such as PKN2. Is a negative regulator of ciliogenesis through phosphorylation of PKN2 and promotion of RhoA signaling.

1. Guen, VJ. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 19525-30. PMID: 24218572
2. Guen, VJ. et al. (2016) Cell cycle (Georgetown, Tex.) 15, 678-88. PMID: 27104747
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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