Anti-CEBPA Polyclonal Antibody (HW834024)

Name: Anti-CEBPA Polyclonal Antibody
Brand: abinScience
SKU: HW834024
Price: 168 USD
Availability: InStock

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Aperçu

Numéro de catalogue

HW834024

Description

Anti-CEBPA Polyclonal Antibody (HW834024) is a rabbit polyclonal antibody detecting CCAAT/enhancer-binding protein alpha; C/EBP alpha; CEBPA; CEBP in ELISA, IHC, WB. Suitable for Mouse, Bovine, Rat, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant human CEBPA (Asn281-Ala358).

Cible

CCAAT/enhancer-binding protein alpha; C/EBP alpha; CEBPA; CEBP

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P53566, O02754, P05554, P49715

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

CCAAT/enhancer-binding protein alpha (CEBPA) is a ~37 kDa protein. Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes. During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung.

1. Wang, GL. et al. (2004) Genes & development 18, 912-25. PMID: 15107404

2. Zhang, P. et al. (2004) Immunity 21, 853-63. PMID: 15589173

3. Zhang, Z. et al. (2022) Cell metabolism 34, 1860-1874.e4. PMID: 36228616

4. Lin, FT. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 9606-10. PMID: 8415748

5. Bégay, V. et al. (2004) Molecular and cellular biology 24, 9744-51. PMID: 15509779

6. Ohlsson, E. et al. (2014) The Journal of experimental medicine 211, 5-13. PMID: 24367003

7. Flodby, P. et al. (1996) The Journal of biological chemistry 271, 24753-60. PMID: 8798745

8. Samuelsson, L. et al. (1991) The EMBO journal 10, 3787-93. PMID: 1935900

9. Lin, FT. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 8757-61. PMID: 8090719

10. Porse, BT. et al. (2001) Cell 107, 247-58. PMID: 11672531

Note

For research use only

Images

Reférences

Fiche technique

Anti-CEBPA Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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