Anti-CFL1 Polyclonal Antibody (HB213014)

Name: Anti-CFL1 Polyclonal Antibody
Brand: abinScience
SKU: HB213014
Price: 96 USD
Availability: InStock

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Especificación:

20ug
50ug
100ug

Número:

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Número de catálogo

HB213014

Descripción

Anti-CFL1 Polyclonal Antibody (HB213014) is a rabbit polyclonal antibody detecting CFL1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human CFL1 (Ser3-Leu161).

Objetivo

Cofilin, non-muscle isoform, CFL1, p18, 18 kDa phosphoprotein, CFL, Cofilin-1

Purificación

Purified by antigen affinity column.

Número de acceso

P23528

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Cofilin-1 (CFL1) is a ~18 kDa protein. Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the centralization of the mitotic spindle and symmetric division of zygotes. Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.

1. Yeoh, S. et al. (2002) Journal of molecular biology 315, 911-25. PMID: 11812157

2. Gohla, A. et al. (2005) Nature cell biology 7, 21-9. PMID: 15580268

3. Bai, SW. et al. (2011) BMC biology 9, 54. PMID: 21834987

4. Borroni, EM. et al. (2013) Science signaling 6, ra30.1-11, S1-3. PMID: 23633677

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-CFL1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: H1299 cell lysate
Lane 4: Hela cell lysate
Lane 5: Jurkat cell lysate
Lane 6: SH-SY5Y cell lysate
Lane 7: PC-12 cell lysate
Lane 8: Mouse brain lysate
Lane 9: Rat brain lysate

Referencias

Datasheet

Anti-CFL1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-CFL1 Polyclonal Antibody (HB213014)

Vista general

Imágenes

Referencias

Datasheet

SDS