Anti-CFL1 Polyclonal Antibody (HB213014)

Name: Anti-CFL1 Polyclonal Antibody
Brand: abinScience
SKU: HB213014
Price: 96 USD
Availability: InStock

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Каталожный номер

HB213014

Описание

Anti-CFL1 Polyclonal Antibody (HB213014) is a rabbit polyclonal antibody detecting CFL1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human CFL1 (Ser3-Leu161).

Цель

Cofilin, non-muscle isoform, CFL1, p18, 18 kDa phosphoprotein, CFL, Cofilin-1

Очистка

Purified by antigen affinity column.

Номер доступа

P23528

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Cofilin-1 (CFL1) is a ~18 kDa protein. Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the centralization of the mitotic spindle and symmetric division of zygotes. Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.

1. Yeoh, S. et al. (2002) Journal of molecular biology 315, 911-25. PMID: 11812157

2. Gohla, A. et al. (2005) Nature cell biology 7, 21-9. PMID: 15580268

3. Bai, SW. et al. (2011) BMC biology 9, 54. PMID: 21834987

4. Borroni, EM. et al. (2013) Science signaling 6, ra30.1-11, S1-3. PMID: 23633677

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-CFL1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: H1299 cell lysate
Lane 4: Hela cell lysate
Lane 5: Jurkat cell lysate
Lane 6: SH-SY5Y cell lysate
Lane 7: PC-12 cell lysate
Lane 8: Mouse brain lysate
Lane 9: Rat brain lysate

Ссылки

Даташит

Anti-CFL1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-CFL1 Polyclonal Antibody (HB213014)

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