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Anti-CFL2 Polyclonal Antibody (HV067014)

Anti-CFL2 Polyclonal Antibody
Anti-CFL2 Polyclonal Antibody
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  • 20ug
  • 50ug
  • 100ug
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Vista general
Número de catálogoHV067014
Descripción
Anti-CFL2 Polyclonal Antibody (HV067014) is a rabbit polyclonal antibody detecting CFL2 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human CFL2 (Ala2-Leu166).
Objetivo CFL2, Cofilin-2, Cofilin, muscle isoform
Purificación Purified by antigen affinity column.
Número de acceso Q9Y281
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Cofilin-2 (CFL2) is a ~18 kDa protein. Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance.

1. Papalouka, V. et al. (2009) Molecular and cellular biology 29, 6046-58. PMID: 19752190
Nota For research use only.
Imágenes
  • Anti-CFL2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CFL2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Raji cell lysate
    Lane 2: Mouse brain lysate
    Lane 3: Mouse lung lysate
    Lane 4: Rat brain lysate
    Lane 5: Rat lung lysate
    Lane 6: Rat skeletal muscle lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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