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Anti-CFL2 Polyclonal Antibody (HV067014)

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Anti-CFL2 Polyclonal Antibody
Anti-CFL2 Polyclonal Antibody
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Aperçu
Numéro de catalogueHV067014
Description
Anti-CFL2 Polyclonal Antibody (HV067014) is a rabbit polyclonal antibody detecting CFL2 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human CFL2 (Ala2-Leu166).
Cible CFL2, Cofilin-2, Cofilin, muscle isoform
Purification Purified by antigen affinity column.
Numéro d'accès Q9Y281
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Cofilin-2 (CFL2) is a ~18 kDa protein. Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance.

1. Papalouka, V. et al. (2009) Molecular and cellular biology 29, 6046-58. PMID: 19752190
Note For research use only.
Images
  • Anti-CFL2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CFL2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Raji cell lysate
    Lane 2: Mouse brain lysate
    Lane 3: Mouse lung lysate
    Lane 4: Rat brain lysate
    Lane 5: Rat lung lysate
    Lane 6: Rat skeletal muscle lysate

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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Anti-CFL2 Polyclonal Antibody

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