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Anti-CFL2 Polyclonal Antibody (HV067014)

Anti-CFL2 Polyclonal Antibody
Anti-CFL2 Polyclonal Antibody
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概要
カタログ番号HV067014
説明
Anti-CFL2 Polyclonal Antibody (HV067014) is a rabbit polyclonal antibody detecting CFL2 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
種反応性Human, Mouse
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Human CFL2 (Ala2-Leu166).
ターゲット CFL2, Cofilin-2, Cofilin, muscle isoform
精製 Purified by antigen affinity column.
アクセッション番号 Q9Y281
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Cofilin-2 (CFL2) is a ~18 kDa protein. Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance.

1. Papalouka, V. et al. (2009) Molecular and cellular biology 29, 6046-58. PMID: 19752190
注意事項 For research use only.
イメージ
  • Anti-CFL2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CFL2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Raji cell lysate
    Lane 2: Mouse brain lysate
    Lane 3: Mouse lung lysate
    Lane 4: Rat brain lysate
    Lane 5: Rat lung lysate
    Lane 6: Rat skeletal muscle lysate

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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