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Anti-CHIKV Capsid Protein Polyclonal Antibody (VK572024)

Anti-CHIKV Capsid Protein Polyclonal Antibody
Anti-CHIKV Capsid Protein Polyclonal Antibody
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Aperçu
Numéro de catalogueVK572024
Description
Anti-CHIKV Capsid Protein Polyclonal Antibody (VK572024) is a rabbit polyclonal antibody detecting Capsid protein in ELISA, IHC, WB. Suitable for Chikungunya virus, O'nyong-nyong virus, Middelburg virus, Ross river virus, and Sagiyama virus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesChikungunya virus (CHIKV)
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant CHIKV Capsid Protein (Arg104-Trp261).
Cible C, Capsid protein, Coat protein
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Numéro d'accès P0DOK1, Q8JUX5, Q5WQY5, Q5XXP3, O90371, P22056, O90369, P03315, P0DJZ6, Q80S27, P08491, Q9JGK8, Q5Y388, P13890, Q8QZ72
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Frameshifted structural polyprotein is a ~92 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

Note For research use only
Images
  • Anti-CHIKV Capsid Protein Polyclonal Antibody

    Western blot

    Lane 1: recombinant CHIKV Capsid (Catalog No: VK572022)

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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