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Anti-CHIKV Capsid Protein Polyclonal Antibody (VK572024)

Anti-CHIKV Capsid Protein Polyclonal Antibody
Anti-CHIKV Capsid Protein Polyclonal Antibody
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概要
カタログ番号VK572024
説明
Anti-CHIKV Capsid Protein Polyclonal Antibody (VK572024) is a rabbit polyclonal antibody detecting Capsid protein in ELISA, IHC, WB. Suitable for Chikungunya virus, O'nyong-nyong virus, Middelburg virus, Ross river virus, and Sagiyama virus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
種反応性Chikungunya virus (CHIKV)
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant CHIKV Capsid Protein (Arg104-Trp261).
ターゲット C, Capsid protein, Coat protein
エンドトキシンレベル Please contact with the lab for this information.
純度 >95% as determined by SDS-PAGE.
精製 Purified by antigen affinity column.
アクセッション番号 P0DOK1, Q8JUX5, Q5WQY5, Q5XXP3, O90371, P22056, O90369, P03315, P0DJZ6, Q80S27, P08491, Q9JGK8, Q5Y388, P13890, Q8QZ72
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

発送 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Frameshifted structural polyprotein is a ~92 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

注意事項 For research use only
イメージ
  • Anti-CHIKV Capsid Protein Polyclonal Antibody

    Western blot

    Lane 1: recombinant CHIKV Capsid (Catalog No: VK572022)

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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