Anti-CHIKV Spike glycoprotein E2 Recombinant Antibody (4J21) (VK572033)

Name: Anti-CHIKV Spike glycoprotein E2 Recombinant Antibody (4J21)
Brand: abinScience
SKU: VK572033
Price: 126 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

VK572033

Reactivité des espèces

Chikungunya virus (CHIKV)

Applications

ELISA, WB

Espèce hôte

Human

Clonalité

Monoclonal

Isotype

IgG1, kappa

ID de clone

4J21

Cible

Structural polyprotein, Envelope glycoprotein E2, Spike glycoprotein E2

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q8JUX5

RRID

Anti-CHIKV Spike glycoprotein E2 Antibody (4J21) (abinScience Cat# VK572033, RRID:AB_3725325)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Structural polyprotein (Spike glycoprotein E2) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Webb, LG. et al. (2020) PLoS pathogens 16, e1008999. PMID: 33057424

Note

For research use only.

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