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| カタログ番号 | VK572043 | ||||||
|---|---|---|---|---|---|---|---|
| 種反応性 | Chikungunya virus (CHIKV) | ||||||
| アプリケーション | ELISA, IF, WB | ||||||
| 宿主種 | Human | ||||||
| クローン性 | Monoclonal | ||||||
| アイソタイプ | IgG1, kappa | ||||||
| クローンID | 5F10 | ||||||
| ターゲット | Structural polyprotein, Envelope glycoprotein E2, Spike glycoprotein E2 | ||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||
| 純度 | >95% as determined by SDS-PAGE. | ||||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||||
| アクセッション番号 | Q8JUX5, Q5XXP3, Q5WQY5 | ||||||
| RRID | Anti-CHIKV Spike glycoprotein E2 Antibody (5F10) (abinScience Cat# VK572043, RRID:AB_3725326) |
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| 形態 | Liquid | ||||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| 背景 | Structural polyprotein (Spike glycoprotein E2) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions. 1. Webb, LG. et al. (2020) PLoS pathogens 16, e1008999. PMID: 33057424 | ||||||
| 注意事項 | For research use only. |
SDS-PAGE for Anti-CHIKV Spike glycoprotein E2 Antibody (5F10)
Detects recombinant CHIKV Spike glycoprotein E2 (Catalog No: VK572021) in indirect ELISA.
Western blot analysis was performed using anti-CHIKV E2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant CHIKV Spike glycoprotein E2 (Catalog No: VK572021)
Lane 2: negative control
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