Anti-CHIKV Spike glycoprotein E2 Recombinant Antibody (5F10) (VK572043)

Name: Anti-CHIKV Spike glycoprotein E2 Recombinant Antibody (5F10)
Brand: abinScience
SKU: VK572043
Price: 126 USD
Availability: InStock

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Цена(USD):

Спецификация:

20ug
50ug
100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

VK572043

Реактивность видов

Chikungunya virus (CHIKV)

Применения

ELISA, IF, WB

Хозяинский вид

Human

Клоональность

Monoclonal

Изотип

IgG1, kappa

ID клона

5F10

Цель

Structural polyprotein, Envelope glycoprotein E2, Spike glycoprotein E2

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

Q8JUX5, Q5XXP3, Q5WQY5

RRID

Anti-CHIKV Spike glycoprotein E2 Antibody (5F10) (abinScience Cat# VK572043, RRID:AB_3725326)

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Фон

Structural polyprotein (Spike glycoprotein E2) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Webb, LG. et al. (2020) PLoS pathogens 16, e1008999. PMID: 33057424

Примечание

For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Anti-CHIKV Spike glycoprotein E2 Antibody (5F10)
Bioactivity

Detects recombinant CHIKV Spike glycoprotein E2 (Catalog No: VK572021) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-CHIKV E2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant CHIKV Spike glycoprotein E2 (Catalog No: VK572021)
Lane 2: negative control