Anti-CHIKV Spike glycoprotein E2 Polyclonal Antibody (VK572014)

Name: Anti-CHIKV Spike glycoprotein E2 Polyclonal Antibody
Brand: abinScience
SKU: VK572014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

VK572014

Description

Anti-CHIKV Spike glycoprotein E2 Polyclonal Antibody (VK572014) is a rabbit polyclonal antibody detecting Spike glycoprotein E2 in ELISA, IHC, WB. Suitable for Chikungunya virus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Chikungunya virus (CHIKV)

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant CHIKV Structural polyprotein (Asn332-Leu667).

Cible

Structural polyprotein, p130, Precursor of protein E3/E2, p62, pE2, Spike glycoprotein E2, E2 envelope glycoprotein

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

Q8JUX5, P0DOK1, Q5WQY5, Q5XXP3

RRID

Anti-CHIKV Spike glycoprotein E2 Polyclonal Antibody (abinScience Cat# VK572014, RRID:AB_3751765)

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Structural polyprotein (E2) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Webb, LG. et al. (2020) PLoS pathogens 16, e1008999. PMID: 33057424

Note

For research use only

Images

Reférences

Fiche technique

Anti-CHIKV Spike glycoprotein E2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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