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Anti-CHMP4B Polyclonal Antibody (HB565014)

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Aperçu
Numéro de catalogueHB565014
Description
Anti-CHMP4B Polyclonal Antibody (HB565014) is a rabbit polyclonal antibody detecting Charged multivesicular body protein 4b; Chromatin-modifying protein 4b; CHMP4b; SNF7 homolog associated with Alix 1; SNF7-2; hSnf7-2; Vacuolar protein sorting-associated protein 32-2; Vps32-2; hVps32-2; CHMP4B; C20orf178; SHAX1 in ELISA, IHC, WB. Suitable for Human, Mouse, Chicken, and Frog.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant human CHMP4B (Asp121-Met224).
Cible Charged multivesicular body protein 4b; Chromatin-modifying protein 4b; CHMP4b; SNF7 homolog associated with Alix 1; SNF7-2; hSnf7-2; Vacuolar protein sorting-associated protein 32-2; Vps32-2; hVps32-2; CHMP4B; C20orf178; SHAX1
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q9H444, Q9D8B3, P59074, Q5ZHP5, Q5XGW6
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Charged multivesicular body protein 4b (CHMP4B) is a ~24 kDa protein. Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis.

1. Katoh, K. et al. (2003) The Journal of biological chemistry 278, 39104-13. PMID: 12860994
2. Hanson, PI. et al. (2008) The Journal of cell biology 180, 389-402. PMID: 18209100
3. Guizetti, J. et al. (2011) Science (New York, N.Y.) 331, 1616-20. PMID: 21310966
4. Vietri, M. et al. (2015) Nature 522, 231-5. PMID: 26040712
5. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413
6. Baeumers, M. et al. (2020) BMC biology 18, 200. PMID: 33349255
Note For research use only
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Formula
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C₂ (Working Conc.)
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Anti-CHMP4B Polyclonal Antibody

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