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Anti-CILK1 Polyclonal Antibody (HV486014)

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Vista general
Número de catálogoHV486014
Descripción
Anti-CILK1 Polyclonal Antibody (HV486014) is a rabbit polyclonal antibody detecting CILK1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human CILK1 (Met1-Gln277).
Objetivo Ciliogenesis associated kinase 1, Serine/threonine-protein kinase ICK, MAK-related kinase, ICK, KIAA0936, hICK, Laryngeal cancer kinase 2, CILK1, MRK, LCK2, Intestinal cell kinase
Purificación Purified by antigen affinity column.
Número de acceso Q9UPZ9
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Serine/threonine-protein kinase ICK (CILK1) is a ~71 kDa protein. Required for ciliogenesis. Phosphorylates KIF3A. Involved in the control of ciliary length. Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips. May play a key role in the development of multiple organ systems and particularly in cardiac development.

1. Chaya, T. et al. (2014) The EMBO journal 33, 1227-42. PMID: 24797473
2. Moon, H. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 8541-6. PMID: 24853502
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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