Anti-Clostridioides difficile Toxin A/tcdA Recombinant Nanobody (SAA0992) (JN085023)

Name: Anti-Clostridioides difficile Toxin A/tcdA Recombinant Nanobody (SAA0992)
Brand: abinScience
SKU: JN085023
Price: 126 USD
Availability: InStock

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

JN085023

Описание

Anti-Clostridioides difficile Toxin A/tcdA Nanobody (SAA0992) [SAA0992] (JN085023) is a alpaca monoclonal antibody detecting Clostridium difficile Toxin in ELISA.

Реактивность видов

Clostridioides difficile

Применения

ELISA

Хозяинский вид

Alpaca

Клоональность

Monoclonal

Изотип

VHH-8His-Cys-tag

ID клона

SAA0992

Цель

Toxin A, 3.4.22.-, Glucosyltransferase TcdA, 2.4.1.-, tcdA, toxA, Clostridium difficile Toxin

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Purified by Nickel column.

Номер доступа

P16154

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Toxin A (tcdA) is a ~308 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdA and TcdB constitute the main toxins that mediate the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdB, TcdA is less virulent and less important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdA) into the host cytosol. Targets colonic epithelia by binding to some receptor, and enters host cells via clathrin-mediated endocytosis.

1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489

2. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482

3. Greco, A. et al. (2006) Nature structural & molecular biology 13, 460-1. PMID: 16622409

4. Tucker, KD. et al. (1991) Infection and immunity 59, 73-8. PMID: 1670930

5. Tao, L. et al. (2019) Nature microbiology 4, 1760-1769. PMID: 31160825

6. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706

7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356

8. Pruitt, RN. et al. (2009) The Journal of biological chemistry 284, 21934-21940. PMID: 19553670

Примечание

For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Anti-Clostridioides difficile Toxin A/tcdA Nanobody (SAA0992)

Ссылки

Даташит

Anti-Clostridioides difficile Toxin A/tcdA Recombinant Nanobody (SAA0992).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа