Anti-Clostridioides difficile Toxin A/tcdA Recombinant Nanobody (SAA0993) (JN085013)

Name: Anti-Clostridioides difficile Toxin A/tcdA Recombinant Nanobody (SAA0993)
Brand: abinScience
SKU: JN085013
Price: 126 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

JN085013

Descripción

Anti-Clostridioides difficile Toxin A/tcdA Nanobody (SAA0993) [SAA0993] (JN085013) is a alpaca monoclonal antibody detecting Clostridium difficile Toxin in ELISA.

Reactividad de especies

Clostridioides difficile

Aplicaciones

ELISA, WB

Especie huésped

Alpaca

Clonalidad

Monoclonal

Isotipo

VHH-8His-Cys-tag

ID de clon

SAA0993

Aplicaciones probadas

Objetivo

Toxin A, 3.4.22.-, Glucosyltransferase TcdA, 2.4.1.-, tcdA, toxA, Clostridium difficile Toxin

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Purified by Nickel column.

Número de acceso

P16154

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Toxin A (tcdA) is a ~308 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdA and TcdB constitute the main toxins that mediate the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdB, TcdA is less virulent and less important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdA) into the host cytosol. Targets colonic epithelia by binding to some receptor, and enters host cells via clathrin-mediated endocytosis.

1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489

2. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482

3. Greco, A. et al. (2006) Nature structural & molecular biology 13, 460-1. PMID: 16622409

4. Tucker, KD. et al. (1991) Infection and immunity 59, 73-8. PMID: 1670930

5. Tao, L. et al. (2019) Nature microbiology 4, 1760-1769. PMID: 31160825

6. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706

7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356

8. Pruitt, RN. et al. (2009) The Journal of biological chemistry 284, 21934-21940. PMID: 19553670

Nota

For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Clostridioides difficile Toxin A/tcdA Nanobody (SAA0993)
Bioactivity

Detects Clostridioides difficile Toxin A (Catalog No: JN085012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-Toxin A monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Clostridioides difficil Toxin A (Catalog No: JN085012)