Anti-Clostridioides difficile Toxin A/tcdA Recombinant Nanobody (SAA0993) (JN085013)

Name: Anti-Clostridioides difficile Toxin A/tcdA Recombinant Nanobody (SAA0993)
Brand: abinScience
SKU: JN085013
Price: 126 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

JN085013

Description

Anti-Clostridioides difficile Toxin A/tcdA Nanobody (SAA0993) [SAA0993] (JN085013) is a alpaca monoclonal antibody detecting Clostridium difficile Toxin in ELISA.

Reactivité des espèces

Clostridioides difficile

Applications

ELISA, WB

Espèce hôte

Alpaca

Clonalité

Monoclonal

Isotype

VHH-8His-Cys-tag

ID de clone

SAA0993

Applications testées

Cible

Toxin A, 3.4.22.-, Glucosyltransferase TcdA, 2.4.1.-, tcdA, toxA, Clostridium difficile Toxin

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Numéro d'accès

P16154

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Toxin A (tcdA) is a ~308 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdA and TcdB constitute the main toxins that mediate the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdB, TcdA is less virulent and less important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdA) into the host cytosol. Targets colonic epithelia by binding to some receptor, and enters host cells via clathrin-mediated endocytosis.

1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489

2. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482

3. Greco, A. et al. (2006) Nature structural & molecular biology 13, 460-1. PMID: 16622409

4. Tucker, KD. et al. (1991) Infection and immunity 59, 73-8. PMID: 1670930

5. Tao, L. et al. (2019) Nature microbiology 4, 1760-1769. PMID: 31160825

6. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706

7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356

8. Pruitt, RN. et al. (2009) The Journal of biological chemistry 284, 21934-21940. PMID: 19553670

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Clostridioides difficile Toxin A/tcdA Nanobody (SAA0993)
Bioactivity

Detects Clostridioides difficile Toxin A (Catalog No: JN085012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-Toxin A monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Clostridioides difficil Toxin A (Catalog No: JN085012)