Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0941) (JN959053)

Name: Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0941)
Brand: abinScience
SKU: JN959053
Price: 126 USD
Availability: InStock

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20ug
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1mg

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Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

JN959053

Descripción

Anti-Clostridium botulinum BoNT/B Nanobody (SAA0941) [SAA0941] (JN959053) is a alpaca monoclonal antibody detecting BoNT/B in ELISA.

Reactividad de especies

Clostridium botulinum

Aplicaciones

ELISA

Especie huésped

Alpaca

Clonalidad

Monoclonal

Isotipo

VHH-8His-Cys-tag

ID de clon

SAA0941

Objetivo

Botulinum neurotoxin type B, BoNT/B, Bontoxilysin-B, Botulinum neurotoxin B light chain, LC, 3.4.24.69, Botulinum neurotoxin B heavy chain, HC, botB

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Purified by Nickel column.

Número de acceso

P10844

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Botulinum neurotoxin type B (BoNT) is a ~150 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin B which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

1. Chai, Q. et al. (2006) Nature 444, 1096-100. PMID: 17167418

2. Jin, R. et al. (2006) Nature 444, 1092-5. PMID: 17167421

3. Rummel, A. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 359-64. PMID: 17185412

4. Berntsson, RP. et al. (2013) Nature communications 4, 2058. PMID: 23807078

5. Dong, M. et al. (2003) The Journal of cell biology 162, 1293-303. PMID: 14504267

6. Hoch, DH. et al. (1985) Proceedings of the National Academy of Sciences of the United States of America 82, 1692-6. PMID: 3856850

7. Nishiki, T. et al. (1994) The Journal of biological chemistry 269, 10498-503. PMID: 8144634

10. Sun, S. et al. (2012) Biochemistry 51, 5655-62. PMID: 22720883

Nota

For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Clostridium botulinum BoNT/B Nanobody (SAA0941)

Referencias

Datasheet

Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0941).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo