Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0941) (JN959053)

Name: Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0941)
Brand: abinScience
SKU: JN959053
Price: 126 USD
Availability: InStock

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50ug
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1mg

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

JN959053

Описание

Anti-Clostridium botulinum BoNT/B Nanobody (SAA0941) [SAA0941] (JN959053) is a alpaca monoclonal antibody detecting BoNT/B in ELISA.

Реактивность видов

Clostridium botulinum

Применения

ELISA

Хозяинский вид

Alpaca

Клоональность

Monoclonal

Изотип

VHH-8His-Cys-tag

ID клона

SAA0941

Цель

Botulinum neurotoxin type B, BoNT/B, Bontoxilysin-B, Botulinum neurotoxin B light chain, LC, 3.4.24.69, Botulinum neurotoxin B heavy chain, HC, botB

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Purified by Nickel column.

Номер доступа

P10844

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Botulinum neurotoxin type B (BoNT) is a ~150 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin B which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

1. Chai, Q. et al. (2006) Nature 444, 1096-100. PMID: 17167418

2. Jin, R. et al. (2006) Nature 444, 1092-5. PMID: 17167421

3. Rummel, A. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 359-64. PMID: 17185412

4. Berntsson, RP. et al. (2013) Nature communications 4, 2058. PMID: 23807078

5. Dong, M. et al. (2003) The Journal of cell biology 162, 1293-303. PMID: 14504267

6. Hoch, DH. et al. (1985) Proceedings of the National Academy of Sciences of the United States of America 82, 1692-6. PMID: 3856850

7. Nishiki, T. et al. (1994) The Journal of biological chemistry 269, 10498-503. PMID: 8144634

10. Sun, S. et al. (2012) Biochemistry 51, 5655-62. PMID: 22720883

Примечание

For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Anti-Clostridium botulinum BoNT/B Nanobody (SAA0941)

Ссылки

Даташит

Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0941).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа