Anti-Clostridium botulinum BoNT/E Recombinant Antibody (SAA2610) (JN161013)

Name: Anti-Clostridium botulinum BoNT/E Recombinant Antibody (SAA2610)
Brand: abinScience
SKU: JN161013
Price: 191 USD
Availability: InStock

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50ug
100ug
1mg

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Reférences
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SDS

Aperçu

Numéro de catalogue

JN161013

Reactivité des espèces

Clostridium botulinum

Applications

ELISA

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a, kappa

ID de clone

SAA2610

Cible

BoNT/E, Bontoxilysin-E, Botulinum neurotoxin E heavy chain, Botulinum neurotoxin E light chain, Botulinum neurotoxin type E, EC:3.4.24.69, HC, LC, botE

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q00496

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Botulinum neurotoxin type E (BoNT) is a ~143 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin E which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

1. Fu, Z. et al. (2009) Biochemistry 48, 5631-41. PMID: 19476346

2. Rummel, A. et al. (2009) Journal of neurochemistry 110, 1942-54. PMID: 19650874

3. Sun, S. et al. (2012) Biochemistry 51, 5655-62. PMID: 22720883

4. Dong, M. et al. (2008) Molecular biology of the cell 19, 5226-37. PMID: 18815274

Note

For research use only

Images

Reférences

Fiche technique

Anti-Clostridium botulinum BoNT/E Recombinant Antibody (SAA2610).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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