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| カタログ番号 | JN888243 | ||||
|---|---|---|---|---|---|
| 種反応性 | Clostridium botulinum | ||||
| アプリケーション | ELISA | ||||
| 宿主種 | Mouse | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | IgG2a | ||||
| クローンID | 1F3 | ||||
| ターゲット | botA, atx, bonT, Botulinum neurotoxin type A, BoNT/A, Bontoxilysin-A, BOTOX, Botulinum neurotoxin type A1 | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||
| アクセッション番号 | P0DPI0 | ||||
| RRID | Anti-Clostridium botulinum botA/BoNT Antibody (1F3) (abinScience Cat# JN888243, RRID:AB_3727090) |
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| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 背景 | Botulinum neurotoxin type A (botA/BoNT) is a ~149 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol. 1. BURGEN, AS. et al. (1949) The Journal of physiology 109, 10-24. PMID: 15394302 2. Zhou, L. et al. (1995) Biochemistry 34, 15175-81. PMID: 7578132 3. Fischer, A. et al. (2007) The Journal of biological chemistry 282, 29604-11. PMID: 17666397 4. Fischer, A. et al. (2008) PLoS pathogens 4, e1000245. PMID: 19096517 5. Yowler, BC. et al. (2002) The Journal of biological chemistry 277, 32815-9. PMID: 12089155 6. Dong, M. et al. (2006) Science (New York, N.Y.) 312, 592-6. PMID: 16543415 7. Mahrhold, S. et al. (2006) FEBS letters 580, 2011-4. PMID: 16545378 8. Dong, M. et al. (2008) Molecular biology of the cell 19, 5226-37. PMID: 18815274 | ||||
| 注意事項 | For research use only. |
SDS-PAGE for Anti-Clostridium botulinum botA/BoNT Antibody (1F3).
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