Anti-Clostridium botulinum botA/BOTOX Recombinant Antibody (VH22) (JN888223)

Name: Anti-Clostridium botulinum botA/BOTOX Recombinant Antibody (VH22)
Brand: abinScience
SKU: JN888223
Price: 126 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

JN888223

Reactivité des espèces

Clostridium botulinum

Applications

ELISA, WB

Espèce hôte

Human

Clonalité

Monoclonal

Isotype

VHH-Fc

ID de clone

VH22

Cible

botA, atx, bonT, Botulinum neurotoxin type A, BoNT/A, Bontoxilysin-A, BOTOX, Botulinum neurotoxin type A1

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

P0DPI0

RRID

Anti-Clostridium botulinum botA/BoNT Antibody (VH22) (abinScience Cat# JN888223, RRID:AB_3727088)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Botulinum neurotoxin type A (botA/BoNT) is a ~149 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

1. BURGEN, AS. et al. (1949) The Journal of physiology 109, 10-24. PMID: 15394302

2. Zhou, L. et al. (1995) Biochemistry 34, 15175-81. PMID: 7578132

3. Fischer, A. et al. (2007) The Journal of biological chemistry 282, 29604-11. PMID: 17666397

4. Fischer, A. et al. (2008) PLoS pathogens 4, e1000245. PMID: 19096517

5. Yowler, BC. et al. (2002) The Journal of biological chemistry 277, 32815-9. PMID: 12089155

6. Dong, M. et al. (2006) Science (New York, N.Y.) 312, 592-6. PMID: 16543415

7. Mahrhold, S. et al. (2006) FEBS letters 580, 2011-4. PMID: 16545378

8. Dong, M. et al. (2008) Molecular biology of the cell 19, 5226-37. PMID: 18815274

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Clostridium botulinum botA/BoNT Antibody (VH22).

Reférences

Fiche technique

Anti-Clostridium botulinum botA/BOTOX Recombinant Antibody (VH22).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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