Anti-Clostridium botulinum botA/BOTOX Recombinant Antibody (VHH17) (JN888233)

Name: Anti-Clostridium botulinum botA/BOTOX Recombinant Antibody (VHH17)
Brand: abinScience
SKU: JN888233
Price: 126 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

JN888233

Reactividad de especies

Clostridium botulinum

Aplicaciones

ELISA, WB

Especie huésped

Human

Clonalidad

Monoclonal

Isotipo

VHH-Fc

ID de clon

VHH17

Objetivo

botA, atx, bonT, Botulinum neurotoxin type A, BoNT/A, Bontoxilysin-A, BOTOX, Botulinum neurotoxin type A1

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P0DPI0

RRID

Anti-Clostridium botulinum botA/BoNT Antibody (VHH17) (abinScience Cat# JN888233, RRID:AB_3727089)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

Botulinum neurotoxin type A (botA/BoNT) is a ~149 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

1. BURGEN, AS. et al. (1949) The Journal of physiology 109, 10-24. PMID: 15394302

2. Zhou, L. et al. (1995) Biochemistry 34, 15175-81. PMID: 7578132

3. Fischer, A. et al. (2007) The Journal of biological chemistry 282, 29604-11. PMID: 17666397

4. Fischer, A. et al. (2008) PLoS pathogens 4, e1000245. PMID: 19096517

5. Yowler, BC. et al. (2002) The Journal of biological chemistry 277, 32815-9. PMID: 12089155

6. Dong, M. et al. (2006) Science (New York, N.Y.) 312, 592-6. PMID: 16543415

7. Mahrhold, S. et al. (2006) FEBS letters 580, 2011-4. PMID: 16545378

8. Dong, M. et al. (2008) Molecular biology of the cell 19, 5226-37. PMID: 18815274

Nota

For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Clostridium botulinum botA/BoNT Antibody (VHH17).

Referencias

Datasheet

Anti-Clostridium botulinum botA/BOTOX Recombinant Antibody (VHH17).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo