Anti-Clostridium botulinum botA/BOTOX Recombinant Nanobody (SAA0925) (JN888053)

Name: Anti-Clostridium botulinum botA/BOTOX Recombinant Nanobody (SAA0925)
Brand: abinScience
SKU: JN888053
Price: 191 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug
1mg

Número:

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Vista general
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Referencias
Datasheet
SDS

Vista general

Número de catálogo

JN888053

Descripción

Anti-Clostridium botulinum botA/BOTOX Nanobody (SAA0925) [SAA0925] (JN888053) is a alpaca monoclonal antibody detecting BOTOX in ELISA.

Reactividad de especies

Clostridium botulinum

Aplicaciones

ELISA

Especie huésped

Alpaca

Clonalidad

Monoclonal

Isotipo

VHH-8His-Cys-tag

ID de clon

SAA0925

Objetivo

Botulinum neurotoxin type A, BoNT/A, Bontoxilysin-A, BOTOX, Botulinum neurotoxin A light chain, LC, 3.4.24.69, Botulinum neurotoxin A heavy chain, HC, botA, bna, CBO0806, CLC_0862

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Purified by Nickel column.

Número de acceso

P0DPI1

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Botulinum neurotoxin type A (botA/BOTOX) is a ~149 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

1. Blasi, J. et al. (1993) Nature 365, 160-3. PMID: 8103915

2. Fu, Z. et al. (2009) Biochemistry 48, 5631-41. PMID: 19476346

Nota

For research use only.

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