Anti-Clostridium botulinum botA/BOTOX Recombinant Nanobody (SAA0932) (JN888123)

Name: Anti-Clostridium botulinum botA/BOTOX Recombinant Nanobody (SAA0932)
Brand: abinScience
SKU: JN888123
Price: 191 USD
Availability: InStock

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50ug
100ug
1mg

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Fiche technique
SDS

Aperçu

Numéro de catalogue

JN888123

Description

Anti-Clostridium botulinum botA/BOTOX Nanobody (SAA0932) [SAA0932] (JN888123) is a alpaca monoclonal antibody detecting BOTOX in ELISA.

Reactivité des espèces

Clostridium botulinum

Applications

ELISA

Espèce hôte

Alpaca

Clonalité

Monoclonal

Isotype

VHH-8His-Cys-tag

ID de clone

SAA0932

Cible

Botulinum neurotoxin type A, BoNT/A, Bontoxilysin-A, BOTOX, Botulinum neurotoxin A light chain, LC, 3.4.24.69, Botulinum neurotoxin A heavy chain, HC, botA, bna, CBO0806, CLC_0862

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Numéro d'accès

P0DPI1

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Botulinum neurotoxin type A (botA/BOTOX) is a ~149 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

1. Blasi, J. et al. (1993) Nature 365, 160-3. PMID: 8103915

2. Fu, Z. et al. (2009) Biochemistry 48, 5631-41. PMID: 19476346

Note

For research use only.

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